Reporter

Part:BBa_K316011:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-26)


Reverse strand coding XylE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 437
    Illegal NgoMIV site found at 609
    Illegal AgeI site found at 86
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was designed using several rounds of PCR, using Pfu polymerase to avoid mutations. For more information, please check our wiki[http://2010.igem.org/Team:Imperial_College_London/Strategy].


Source

This part is a modified existing biobrick BBa_J33204.

References